|
KMID : 0370220110550010039
|
|
Yakhak Hoeji
2011 Volume.55 No. 1 p.39 ~ p.44
|
|
|
Antitumor Effect of -Glycyrrhetinic Acid against Human Tumor Xenografts Caused by A549 Cancer Cell
|
|
Kim Ha-Yan
Kim Song-Yi
Lee Jue-Hee
Han Yong-Moon
|
|
|
Abstract
|
|
|
|
Many reports indicate that 18β-glycyrrhetinic acid (18β-GA) from Glycyrrhizae Radix has anti-inflammatory and immunoregulatory activities, whereas reports regarding anticancer activity of the compound are few. In present study, we investigated antitumor effect of 18β-GA on tumor caused by A549 cancer cell in mice. Data resulting from the cytotoxicity assay showed that 18β-GA caused killing of A549 cells. LD?? values of 18β-GA were app. 180㎛ and 80㎛ , corresponding to 48 hr- and 72 hr-treatments, displaying that the killing activity was more effective as the 18β-GA treatment was prolonged. Based on these data, antitumor effect of 18β-GA was tested in nude mice. For induction of the tumor, A549 (3×10? cells/mouse) was injected subcutaneously into the lateral abdomen of nude mice (Balb/c nu/nu). To determine the antitumor effect, nude mice with tumor were given 18β-GA (1 mg/200㎕ /mouse) intraperitoneally every three days for four times. Tumor-sizes were measured with a caliper for a period of 24 days. Results showed that the 18β-GA treatment reduced the tumor-sizes (P<0.05) as compared with negative control nude mice that received diluent (DPBS). The reduction degree was greater than reduction degree by doxorubicin (60㎍ /mouse), and the pattern of reduction was almost sustained during the entire period of the observation. In conclusion, our studies demonstrate that 18β-GA has antitumor activity to the A549 cancer cell-caused tumor.
|
|
|
KEYWORD
|
|
|
18β-GA, A549, antitumor effect, nude mice, doxorubicin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|